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Fremstilling av rotte skjelettmuskelhomogenater for nitrat- og nitrittmålinger
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Fremstilling av rotte skjelettmuskelhomogenater for nitrat- og nitrittmålinger

Article DOI: 10.3791/62427-v • 07:19 min • July 29th, 2021
July 29th, 2021 •
*1, *1, 2, 2, 2, 1, 1
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus, University of Exeter
* These authors contributed equally

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Vi presenterer protokoller for tre ulike metoder for homogenisering av fire ulike muskelgrupper av rotte skjelettmuskelvev for å måle og sammenligne nivåene av nitrat og nitritt. Videre sammenligner vi ulike utvalgsvekter for å undersøke om vevsprøvestørrelse påvirker resultatene av homogenisering.

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Biologi utgave 173 nitrat nitritt nitrogenoksid skjelettmuskulatur vevshomogenisering
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