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Journal / Biology / Laser-Mikrobestrahlung zur Untersuchung der…
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Laser-Mikrobestrahlung zur Untersuchung der DNA-Rekrutierung während der S-Phase
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Laser-Mikrobestrahlung zur Untersuchung der DNA-Rekrutierung während der S-Phase

Article DOI: 10.3791/62466-v • 07:11 min • April 16th, 2021
April 16th, 2021 •
1,2, 1,2,3
1Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 2Laura and Isaac Perlmutter Cancer Center, New York University School of Medicine, 3Howard Hughes Medical Institute, New York University School of Medicine

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Dieses Protokoll beschreibt eine nicht-invasive Methode zur effizienten Identifizierung von S-Phasen-Zellen für nachgelagerte Mikroskopiestudien, wie z. B. die Messung der Rekrutierung von DNA-Reparaturproteinen durch Lasermikrobestrahlung.

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Biologie Ausgabe 170 Mikrobestrahlung S-Phase PCNA-Herde DNA-Schäden Proteinrekrutierung EXO1b 405-nm-Laser
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