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Journal / Biology / 칸디다 알비칸스 Cdr1-mGFPHis 분석을 위한 소규모 플라즈마 멤브레인…
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칸디다 알비칸스 Cdr1-mGFPHis 분석을 위한 소규모 플라즈마 멤브레인 준비
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칸디다 알비칸스 Cdr1-mGFPHis 분석을 위한 소규모 플라즈마 멤브레인 준비

Article DOI: 10.3791/62592-v • 09:44 min • June 13th, 2021
June 13th, 2021 •
*1, *1, 1, 1,2, 3, 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland
* These authors contributed equally

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이 문서는 칸디다 알비칸스 ABC (ATP 결합 카세트) 단백질 Cdr1의 특성화를위한 소규모 플라즈마 막 격리 프로토콜을 제시, 사카로미세스 cerevisiae에서 과발현. 프로테아제-클레이블 C-단말 mGFPCdr1과 태그 사이에 16잔류 링커를 장착한 이중 태그는 Cdr1의 정제 및 세제 스크리닝을 용이하게 하도록 설계되었습니다.

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생물학 제 172
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