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Journal / Genetics / Preparación de muestras para el análisis…
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Genetics
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JoVE Journal Genetics
Preparación de muestras para el análisis bioinformático de la metilación del ADN
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Preparación de muestras para el análisis bioinformático de la metilación del ADN: estrategia de asociación para la obesidad y estudios de rasgos relacionados

Article DOI: 10.3791/62598-v • 14:56 min • May 6th, 2022
May 6th, 2022 •
*1, *1, 1, 2,3, 1, 4, 5, 1, 1, 6, 7, 8, 9, 6
1Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, 2Centre for Computational Biology, University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport, University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, 7Faculty of Pharmaceutical Sciences, University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo
* These authors contributed equally

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El presente estudio describe el flujo de trabajo para gestionar los datos de metilación del ADN obtenidos por las tecnologías de microarrays. El protocolo muestra pasos desde la preparación de la muestra hasta el análisis de datos. Todos los procedimientos se describen en detalle, y el video muestra los pasos significativos.

Tags

Genética Número 183 Metilación del ADN obesidad ChAMP bioinformática epigenética masa grasa índice de masa corporal
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