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Journal / Genetics / Chromosoomscreening van menselijke…
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JoVE Journal Genetics
Chromosoomscreening van menselijke pre-implantatie-embryo's met behulp van gebruikt kweekmedium
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Chromosoomscreening van menselijke pre-implantatie-embryo's met behulp van gebruikt kweekmedium: monsterafname en chromosomale ploïdieanalyse

Article DOI: 10.3791/62619-v • 12:32 min • September 7th, 2021
September 7th, 2021 •
*1,2,3,4, *5, 1,2,3,4, 1,2,3,4, 5, 5, 1,2,3,4, 5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
* These authors contributed equally

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De huidige studie rapporteert een protocol voor chromosoomscreening van menselijke embryo's waarbij gebruik wordt gemaakt van gebruikt kweekmedium, waardoor embryobiopsie wordt vermeden en chromosoomploïdie-identificatie met behulp van NGS mogelijk wordt. Het huidige artikel presenteert de gedetailleerde procedure, inclusief de voorbereiding van kweekmedium, whole genome amplification (WGA), next-generation sequencing (NGS) bibliotheekvoorbereiding en data-analyse.

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Chromosoomscreening Menselijke pre-implantatie-embryo's Gebruikt kweekmedium Monsterafname Chromosomale ploïdieanalyse In-vitrofertilisatie (IVF) Pre-implantatie Genetische Testen voor Aneuploïdie (PGT-A) Trophectoderm (TE) Binnencelmassa (ICM) Valse positieven en negatieven NICS-technologie DNA-sequencing Kweekmediumbemonstering Whole Genome Amplification (WGA) Bibliotheekvoorbereiding NGS-gegevensanalyse Cryopreservatie IVF-laboratorium
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