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Biology

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5-乙炔基-2'-脱氧尿苷/磷酸-组蛋白H3双标记方案用于 果蝇 神经干细胞的细胞周期进展分析
 
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5-乙炔基-2'-脱氧尿苷/磷酸-组蛋白H3双标记方案用于 果蝇 神经干细胞的细胞周期进展分析

Article DOI: 10.3791/62642-v 09:34 min May 4th, 2021
May 4th, 2021

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Summary

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Click here for the English version.

使用5-乙炔基-2'-脱氧尿苷(EdU)和磷酸化组蛋白H3(pH3)标记进行细胞周期分析是一个多步骤程序,可能需要广泛的优化。在这里,我们提出了一个详细的方案,描述了该过程的所有步骤,包括图像分析和定量,以区分不同细胞周期阶段的细胞。

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生物学,第171期,
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