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5-Ethynyl-2'-Deoxyuridine/Fosfo-Histone H3 Dual-Labeling Protocol for cell cycle progresjonsanalyse i Drosophila Neural Stamceller

Article DOI: 10.3791/62642-v • 09:34 min • May 4th, 2021
May 4th, 2021 •
1,2, 1
1Cell Biology, University of Bern, 2Department of life sciences and medicine, University of Luxembourg

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Cellesyklusanalyse med 5-etynyl-2'-deoksyuridin (EdU) og fosfo-histon H3 (pH3) merking er en flertrinnsprosedyre som kan kreve omfattende optimalisering. Her presenterer vi en detaljert protokoll som beskriver alle trinnene for denne prosedyren, inkludert bildeanalyse og kvantifisering for å skille celler i forskjellige cellesyklusfaser.

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