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JoVE Journal Cancer Research
Rumlig profilering af protein- og RNA-ekspression i væv
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Rumlig profilering af protein- og RNA-ekspression i væv: En tilgang til finjustering af virtuel mikrodissektion

Article DOI: 10.3791/62651-v • 09:19 min • July 6th, 2022
July 6th, 2022 •
1, 1, 2, 3, 2, 1
1Department of Pathology, Genentech, 2Department of Microchemistry, Proteomics, Lipidomics & Next Generation Sequencing (MPL-NGS), Genentech, 3NanoString Technologies

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Her beskriver vi en protokol til finjustering af interesseområder (ROI'er) for rumlige omikteknologier for bedre at karakterisere tumormikromiljøet og identificere specifikke cellepopulationer. For proteomics-assays kan automatiserede tilpassede protokoller guide ROI-valg, mens transkriptomics-assays kan finjusteres ved hjælp af ROI'er så små som 50 μm.

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Kræftforskning udgave 185 multiplexing RNA protein mål automatisering ROI visualisering antistof sonder proteomics transkriptomics
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