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JoVE Journal Cancer Research
Romlig profilering av protein- og RNA-uttrykk i vev
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Romlig profilering av protein- og RNA-uttrykk i vev: En tilnærming til finjustering av virtuell mikrodisseksjon

Article DOI: 10.3791/62651-v • 09:19 min • July 6th, 2022
July 6th, 2022 •
1, 1, 2, 3, 2, 1
1Department of Pathology, Genentech, 2Department of Microchemistry, Proteomics, Lipidomics & Next Generation Sequencing (MPL-NGS), Genentech, 3NanoString Technologies

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Her beskriver vi en protokoll for finjustering av interesseområder (ROIs) for Spatial Omics-teknologier for bedre å karakterisere tumormikromiljøet og identifisere spesifikke cellepopulasjoner. For proteomikkanalyser kan automatiserte tilpassede protokoller veilede avkastningsvalg, mens transkriptomikkanalyser kan finjusteres ved å bruke avkastninger så små som 50 μm.

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Cancer Research multipleksing RNA protein mål automatisering ROI visualisering antistoff prober proteomikk transkriptomikk
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