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Journal / Biology / Bakteri hücresi içermeyen Lysate'in Hızlı, Uygun…
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JoVE Journal Biology
Bakteri hücresi içermeyen Lysate'in Hızlı, Uygun Fiyatlı ve Karmaşık Olmayan Üretimi
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Journal (Biology)
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
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Bakteri hücresi içermeyen Lysate'in Hızlı, Uygun Fiyatlı ve Karmaşık Olmayan Üretimi

Article DOI: 10.3791/62753-v • 06:03 min • October 29th, 2021
October 29th, 2021 •
*1, *1,2, *1,3, 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
* These authors contributed equally

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Bu protokol, Escherichia coli'nin tasarlanmış bir suşunu kullanarak ve sadece standart laboratuvar ekipmanı gerektiren hücresiz gen ekspresyözü için bakteriyel lisat üretmek için hızlı ve basit bir yöntemi açıklar.

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