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Ekstracellulære vesikel Optagelse Assay via Confocal Microscope Imaging Analyse
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Ekstracellulære vesikel Optagelse Assay via Confocal Microscope Imaging Analyse

Article DOI: 10.3791/62836-v • 08:32 min • February 14th, 2022
February 14th, 2022 •
*1,2, *1, 1,3, 2,4, 1, 2,4, 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)
* These authors contributed equally

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Ekstracellulære vesikler (elbiler) bidrager til cellulær biologi og intercellulær kommunikation. Der er behov for praktiske analyser til at visualisere og kvantificere elbiler optagelse af cellerne. Den nuværende protokol foreslår EV optagelse assay ved hjælp af tre-dimensionelle fluorescens billeddannelse via konfokal mikroskopi, efter EV isolation af en nano-filtrering-baseret mikrofluidisk enhed.

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Biologi Udgave 180
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