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Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker
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Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

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Denne protokol beskriver en metode til isolering af neutrofiler fra fuldblod, buffy frakker, eller leukaferse membraner, opnå godt udbytte, høj renhed, og minimal celle aktivering. Vi bruger gradient rensning, rød blodlegemer (RBC) sedimentering, og RBC lysis at opnå en høj kvalitet / renhed neutrophil forberedelse.

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Biologi Udgave 175 Neutrophil gradient dextran RBC lysis fuldblod buffy frakke
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