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Journal / Biochemistry / CD95死誘導シグナル複合体の組成とプロスパスパーゼ-8の処理の測定…
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Biochemistry

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JoVE Journal Biochemistry
CD95死誘導シグナル複合体の組成とプロスパスパーゼ-8の処理の測定
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CD95死誘導シグナル複合体の組成とプロスパスパーゼ-8の処理の測定

Article DOI: 10.3791/62842-v • 07:17 min • August 2nd, 2021
August 2nd, 2021 •
1, 1
1Translational Inflammation Research, Center of Dynamic Systems, Otto von Guericke University Magdeburg

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ここでは、死誘導シグナル伝達複合体(DISC)で直接カスパーゼ-8処理を検出し、この複合体の組成を決定する実験的ワークフローを提示する。この方法論は、細胞死経路の分子機構の解明からアポトーシスネットワークの動的モデリングまで幅広い応用を有する。

Tags

生化学、第174号、CD95 DISC形成、免疫沈降、カスパーゼ-8
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