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Biochemistry

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JoVE Journal Biochemistry
Måling sammensætning af CD95 Death-inducerende signalering kompleks og behandling af Procaspase-8 i dette kompleks
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Måling sammensætning af CD95 Death-inducerende signalering kompleks og behandling af Procaspase-8 i dette kompleks

Article DOI: 10.3791/62842-v • 07:17 min • August 2nd, 2021
August 2nd, 2021 •
1, 1
1Translational Inflammation Research, Center of Dynamic Systems, Otto von Guericke University Magdeburg

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Her præsenteres en eksperimentel arbejdsgang, der muliggør påvisning af caspase-8-behandling direkte ved det dødsfremkaldende signalkompleks (DISC) og bestemmer sammensætningen af dette kompleks. Denne metode har brede anvendelser, fra optrævling af de molekylære mekanismer i celledødsveje til dynamisk modellering af apoptosenetværk.

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Biokemi Udgave 174 CD95 DISC dannelse immunprecipitation caspase-8
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