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Journal / Biochemistry / 생체 발광 광유전학 2.0 : 생체 발광을 활용하여 체외 및 생체 내에서 광감각 단백질을 …
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Biochemistry

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JoVE Journal Biochemistry
생체 발광 광유전학 2.0
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
Journal (Biochemistry)
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Journal (Biochemistry)
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
 
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생체 발광 광유전학 2.0 : 생체 발광을 활용하여 체외 및 생체 내에서 광감각 단백질을 활성화 합니다.

Article DOI: 10.3791/62850-v • 07:19 min • August 4th, 2021
August 4th, 2021 •
1,2, 1, 1, 1,2,3
1College of Medicine, Central Michigan University, 2Biochemistry, Cell and Molecular Biology Program, Central Michigan University, 3Neuroscience Program, Central Michigan University

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생체발광-루시퍼라제 효소에 의해 방출되는 광은 소분자 기질인 루시페린-광감각 단백질을 활성화시키기 위해 이용될 수 있고, 이로써 광 자극에 또 다른 차원을 추가하고 시간적 및 공간적 스케일에 걸쳐 세포에서 다수의 광-매개 기능의 조작을 가능하게 한다.

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생화학 문제 174
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