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Automatisere aggregert kvantifisering i Caenorhabditis elegans
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Automatisere aggregert kvantifisering i Caenorhabditis elegans

Article DOI: 10.3791/62997-v • 07:50 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1,2, 1, 1
1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

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Følgende protokoll beskriver utvikling og optimalisering av en arbeidsflyt med høy gjennomstrømning for ormdyrking, fluorescensavbildning og automatisert bildebehandling for å kvantifisere polyglutaminaggregater som en vurdering av endringer i proteostase.

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Biologi utgave 176 C. elegans proteostase polyglutamin aggregering
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