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JoVE Journal Biochemistry
En reporterbasert mobilanalyse for overvåking av skjøteeffektivitet
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En reporterbasert mobilanalyse for overvåking av skjøteeffektivitet

Article DOI: 10.3791/63014-v • 08:53 min • September 15th, 2021
September 15th, 2021 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

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Denne protokollen beskriver en minigene reporteranalyse for å overvåke virkningen av 5'-spleisestedmutasjoner på skjøting og utvikler undertrykkeren U1 snRNA for redning av mutasjonsindusert skjøtehemming. Reporteren og undertrykkeren U1 snRNA-konstruksjoner uttrykkes i HeLa-celler, og skjøting analyseres av primerutvidelse eller RT-PCR.

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Biokjemi Utgave 175 RNA-protein interaksjon pre-mRNA skjøting skjøteosom U1 snRNP intron exon skjøte nettsted Dup51p reporter exon hoppe
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