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Biochemistry

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JoVE Journal Biochemistry
Kontinuerlig fluorescensbasert endonukleasekoblet DNA-metyleringsanalyse for å screene for DNA-metyltransferasehemmere
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Kontinuerlig fluorescensbasert endonukleasekoblet DNA-metyleringsanalyse for å screene for DNA-metyltransferasehemmere

Article DOI: 10.3791/62949-v • 06:07 min • August 5th, 2022
August 5th, 2022 •
1, 2, 2
1Department of Chemistry, Bucknell University, 2Program in Cell Biology/Biochemistry, Bucknell University

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DNA-metyltransferaser er potensielle kreftmedisinmål. Her presenteres en protokoll for å vurdere små molekyler for DNA-metyltransferasehemming. Denne analysen benytter en endonuklease for å koble DNA-metylering til fluorescensgenerering og gjør det mulig å overvåke enzymaktivitet i sanntid.

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