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Journal / Biochemistry / Die Extraktion von Leberglykogenmolekülen zur…
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Biochemistry

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JoVE Journal Biochemistry
Die Extraktion von Leberglykogenmolekülen zur Glykogenstrukturbestimmung
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
Journal (Biochemistry)
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
Microcrystallography of Protein Crystals and In Cellulo Diffraction
Journal (Biochemistry)
Microcrystallography of Protein Crystals and In Cellulo Diffraction
 
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Die Extraktion von Leberglykogenmolekülen zur Glykogenstrukturbestimmung

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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Für die Extraktion von Leberglykogen wurde mittels Saccharosedichtegradientenzentrifugation eine optimale Saccharosekonzentration bestimmt. Die Zugabe eines 10-minütigen Siedeschritts zur Hemmung von Glykogen-abbauenden Enzymen erwies sich als vorteilhaft.

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Diesen Monat in JoVE Ausgabe 180 Glykogenstrukturbestimmung Glukosepolymer Blutzuckerspiegel Eigenschaften von Glykogen Extraktionsmethode Saccharosedichtegradientenzentrifugation molekulare Schäden Saccharoselösungsdichte Glykogenpartikelgrößen Siedeschritt Glykogenabbauende Enzyme denaturieren
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