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Journal / Biochemistry / 글리코겐 구조 측정을 위한 간 글리코겐 분자…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
글리코겐 구조 측정을 위한 간 글리코겐 분자 추출
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Journal (Biochemistry)
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Journal (Biochemistry)
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
 
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글리코겐 구조 측정을 위한 간 글리코겐 분자 추출

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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최적의 슈크로스 농도는 슈크로스 밀도 구배 원심분리를 사용하여 간 글리코겐의 추출을 위해 결정하였다. 글리코겐 분해 효소를 억제하기 위해 10분 끓는 단계를 추가하면 유익한 것으로 입증되었습니다.

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이달의 JoVE 180호 글리코겐 구조 결정 포도당 중합체 혈당 수치 글리코겐의 특성 추출 방법 자당 밀도 구배 원심분리 분자 손상 자당 용액 밀도 글리코겐 입자 크기 끓는 단계 글리코겐 분해 효소 변성
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