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Journal / Developmental Biology / Murine neurale plade målretning af In Utero…
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Developmental Biology

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JoVE Journal Developmental Biology
Murine neurale plade målretning af In Utero nano-injektion (NEPTUN) på embryonal dag 7.5
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Murine neurale plade målretning af In Utero nano-injektion (NEPTUN) på embryonal dag 7.5

Article DOI: 10.3791/63148-v • 10:49 min • February 14th, 2022
February 14th, 2022 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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I denne protokol beskriver vi, hvordan man injicerer musens fosterhule ved E7.5 med lentivirus, hvilket fører til ensartet transduktion af hele neuralpladen med minimale skadelige virkninger på overlevelse eller embryonal udvikling.

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Udviklingsbiologi udgave 180
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