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Journal / Developmental Biology / Focalización de la placa neural murina mediante…
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Developmental Biology

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JoVE Journal Developmental Biology
Focalización de la placa neural murina mediante nanoinyección in utero (NEPTUNE) en el día embrionario 7.5
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Focalización de la placa neural murina mediante nanoinyección in utero (NEPTUNE) en el día embrionario 7.5

Article DOI: 10.3791/63148-v • 10:49 min • February 14th, 2022
February 14th, 2022 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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En este protocolo, describimos cómo inyectar lentivirus la cavidad amniótica del ratón en E7.5, lo que lleva a una transducción uniforme de toda la placa neural, con efectos perjudiciales mínimos sobre la supervivencia o el desarrollo embrionario.

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Biología del desarrollo Número 180
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