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JoVE Journal Developmental Biology
Mikrodissektion og dissociation af Murine-ovidukten
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Mikrodissektion og dissociation af Murine-ovidukten: Individuel segmentidentifikation og enkeltcelleisolering

Article DOI: 10.3791/63168-v • 07:44 min • November 4th, 2021
November 4th, 2021 •
1, 1, 2, 1
1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, 2Microscopy and Imaging Core Facility, University of California, Riverside

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En metode til mikrodissektion af museovidukten, der tillader indsamling af de enkelte segmenter, samtidig med at RNA-integriteten opretholdes, præsenteres. Derudover beskrives ikke-enzymatisk oviduktal celle dissociationsprocedure. Metoderne er egnede til efterfølgende gen- og proteinanalyse af de funktionelt forskellige oviduktale segmenter og dissocierede oviduktale celler.

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Udviklingsbiologi nummer 177
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