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Journal / Developmental Biology / Mikrodissection och dissociation av Murine…
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JoVE Journal Developmental Biology
Mikrodissection och dissociation av Murine Oviduct
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Mikrodissection och dissociation av Murine Oviduct: Individuell segmentidentifiering och isolering av encellsceller

Article DOI: 10.3791/63168-v • 07:44 min • November 4th, 2021
November 4th, 2021 •
1, 1, 2, 1
1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, 2Microscopy and Imaging Core Facility, University of California, Riverside

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En metod för mikrodissection av musovidukten som möjliggör insamling av de enskilda segmenten samtidigt som RNA-integriteten upprätthålls presenteras. Dessutom beskrivs icke-enzymatiska oviductal cell dissociation förfarande. Metoderna är lämpliga för efterföljande gen- och proteinanalys av de funktionellt olika oviduktala segmenten och dissocierade oviduktala celler.

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Utvecklingsbiologi nummer 177
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