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Biologisk forberedelse og mekanisk teknik til bestemmelse af viskoelastiske egenskaber af zonefibre
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Biologisk forberedelse og mekanisk teknik til bestemmelse af viskoelastiske egenskaber af zonefibre

Article DOI: 10.3791/63171-v • 06:39 min • December 16th, 2021
December 16th, 2021 •
1,2, 3, 4, 2,4
1Department of Basic Sciences, University of Health Sciences and Pharmacy, 2Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, 3Department of Biomedical Engineering, The Ohio State University, 4Department of Cell Biology & Physiology, Washington University School of Medicine

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Protokollen beskriver en metode til undersøgelse af ekstracellulær matrixviskoselasticitet og dens afhængighed af proteinsammensætning eller miljøfaktorer. Det målrettede matrixsystem er musezonulen. Metodens ydeevne demonstreres ved at sammenligne den viskoelastiske opførsel af vildtype zonulære fibre med dem, der mangler mikrofibril-associeret glycoprotein-1.

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Medicin udgave 178 ekstracellulær matrix zonule zoneformede fibre viskoelasticitet mikrofibril-associeret glycoprotein-1 trækstyrke elastisk modul stressafslapning kvasi-lineær viskoelastisk model
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