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Journal / Biochemistry / In vitro Triagem de drogas contra todas as etapas …
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Biochemistry

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JoVE Journal Biochemistry
In vitro Triagem de drogas contra todas as etapas do ciclo de vida de Trypanosoma cruzi usando parasitas expressando β-galactosidase
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In vitro Triagem de drogas contra todas as etapas do ciclo de vida de Trypanosoma cruzi usando parasitas expressando β-galactosidase

Article DOI: 10.3791/63210-v • 08:48 min • November 5th, 2021
November 5th, 2021 •
1,2, 1, 1, 3, 1,2, 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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Descrevemos um ensaio colorimétrico de alta produtividade medindo β-galactosidase atividade em três estágios do ciclo de vida de Trypanosoma cruzi, o agente causador da doença de Chagas. Este ensaio pode ser usado para identificar compostos trypanocidas de forma fácil, rápida e reprodutível.

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Bioquímica Edição 177
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