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Journal / Biochemistry / In vitro Caracterização de Chaperones de Histonas …
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Biochemistry

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JoVE Journal Biochemistry
In vitro Caracterização de Chaperones de Histonas utilizando Ensaios Analíticos, Pull-Down e Chaperoning
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In vitro Caracterização de Chaperones de Histonas utilizando Ensaios Analíticos, Pull-Down e Chaperoning

Article DOI: 10.3791/63218-v • 08:16 min • December 29th, 2021
December 29th, 2021 •
*1,2, *1,2, 1,3, 1,2, 1,2, 1, 1,4, 1,5, 1, 1
1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology, KIIT University, 4Department of Molecular Biophysics and Biochemistry, Yale University, 5Department of Pharmacology, University of Vermont College of Medicine
* These authors contributed equally

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Este protocolo descreve uma bateria de métodos que inclui cromatografia analítica de exclusão de tamanho para estudar a oligomerização e estabilidade da histona chaperona, ensaio pull-down para desvendar interações histona-histona-histona, AUC para analisar a estequiometria dos complexos proteicos e ensaio de chaperoning de histonas para caracterizar funcionalmente uma suposta histona chaperone in vitro.

Tags

Bioquímica Edição 178 Chaperona de histona montagem de nucleossomos cromatina nucleoplasmina NAP superenrolamento de plasmídeos ultracentrifugação analítica
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