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Immunology and Infection

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JoVE Journal Immunology and Infection
Generering af nullmutanter for at belyse rollen som bakterielle glycosyltransferaser i bakteriel motilitet
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Generering af nullmutanter for at belyse rollen som bakterielle glycosyltransferaser i bakteriel motilitet

Article DOI: 10.3791/63231-v • 12:29 min • March 11th, 2022
March 11th, 2022 •
1, 2,3, 2,4, 1
1Departamento de Genética, Microbiología y Estadística, Universidad de Barcelona, 2Human Health Therapeutics Research Centre, National Research Council Canada, 3Department of Chemistry, Carleton University, 4Department of Biology, Carleton University

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Her beskriver vi konstruktionen af nulmutanter af Aeromonas i specifikke glycosyltransferaser eller regioner indeholdende glycosyltransferaser, motilitetsassays og flagellarensning udført for at fastslå involvering og funktion af deres kodede enzymer i biosyntesen af en glycan samt denne glycans rolle i bakteriel patogenese.

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Immunologi og infektion udgave 181 O-glycosylering glycosyltransferaser heteroglycan in-frame mutanter mesofile Aeromonas flagelliner flagellarensning CsCl-gradient
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