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Journal / Biology / 使用 体内 抑制、免疫荧光和流式细胞术对精子细胞中驱动蛋白-7 CENP-E…
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使用 体内 抑制、免疫荧光和流式细胞术对精子细胞中驱动蛋白-7 CENP-E 进行功能评估
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
Journal (Biology)
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
Journal (Biology)
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
 
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使用 体内 抑制、免疫荧光和流式细胞术对精子细胞中驱动蛋白-7 CENP-E 进行功能评估

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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本文报道了通过腹部手术和睾丸注射GSK923295对CENP-E的 体内 抑制，这是男性减数分裂的宝贵模型。使用免疫荧光，流式细胞术和透射电子显微镜测定，我们发现CENP-E抑制导致小鼠精子细胞中的染色体错位和基因组不稳定。

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本月在JoVE上，第178期，驱动蛋白，精子细胞，减数分裂，CENP-E，纺锤体，染色体，微管
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