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JoVE Journal Biology
Funktionell bedömning av kinesin-7 CENP-E i spermatocyter med användning av in vivo-hämning, immunofluorescens och flödescytometri
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Funktionell bedömning av kinesin-7 CENP-E i spermatocyter med användning av in vivo-hämning, immunofluorescens och flödescytometri

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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Denna artikel rapporterar en in vivo-hämning av CENP-E genom bukkirurgi och testikelinjektion av GSK923295, en värdefull modell för manlig meiotisk delning. Med hjälp av immunofluorescens-, flödescytometri- och transmissionselektronmikroskopianalyser visar vi att CENP-E-hämning resulterar i kromosomfelinriktning och genominstabilitet i musspermatocyter.

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Denna månad i JoVE nummer 178 kinesin spermatocyt meios CENP-E spindel kromosom mikrotubuli
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