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JoVE Journal
Bioengineering

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Autofluorescence Imaging for å evaluere cellulær metabolisme
 
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Autofluorescence Imaging for å evaluere cellulær metabolisme

Article DOI: 10.3791/63282-v 07:36 min November 15th, 2021
November 15th, 2021

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Summary

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Denne protokollen beskriver fluorescensavbildning og analyse av endogene metabolske koenzymer, redusert nikotinamid adenin (fosfat) dinukleotid (NAD(P)H) og oksidert flavin adenin dinukleotid (FAD). Autofluorescence imaging av NAD (P)H og FAD gir en etikettfri, ikke-ødeleggende metode for å vurdere cellulær metabolisme.

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Bioingeniør Utgave 177 FLIM Metabolisme NAD(P)H FAD Fluorescens Mikroskopi
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