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JoVE Journal Bioengineering
Bioortogonal kjemisk avbildning av cellemetabolisme regulert av aromatiske aminosyrer
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Bioortogonal kjemisk avbildning av cellemetabolisme regulert av aromatiske aminosyrer

Article DOI: 10.3791/65121-v • 10:42 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 1, 1, 1
1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego
* These authors contributed equally

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Vi presenterer en protokoll for direkte å visualisere metabolske aktiviteter i celler regulert av aminosyrer ved bruk av deuteriumoksid (tungtvann D2O) probed stimulert Raman-spredning (DO-SRS) mikroskopi, som er integrert med to-foton eksitasjonsfluorescensmikroskopi (2PEF).

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