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JoVE Journal Bioengineering
Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
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Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Summary
Automatic Translation

This article introduces a simple method for expeditious production of giant unilamellar vesicles with encapsulated cytoskeletal proteins. The method proves to be useful for bottom-up reconstitution of cytoskeletal structures in confinement and cytoskeleton-membrane interactions.

Tags

Encapsulation Reconstituted Cytoskeleton Giant Unilamellar Vesicles Lipid Bilayer In Vitro Reconstitution Synthetic Cell Research Vesicle Generation CDICE Technique Cell-free Transcription-translation Systems Molecular Pathways Minimal Protocells Oil-lipid Mixture Dioleoyl-phosphocholine Cholesterol Rhodamine PE Chloroform Silicone Oil Mineral Oil Vortexing Sonicate
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