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JoVE Journal Bioengineering
Encapsulación rápida del citoesqueleto reconstituido dentro de vesículas unilamelares gigantes
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Encapsulación rápida del citoesqueleto reconstituido dentro de vesículas unilamelares gigantes

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Este artículo presenta un método simple para la producción expedita de vesículas unilamelares gigantes con proteínas citoesqueléticas encapsuladas. El método demuestra ser útil para la reconstitución ascendente de estructuras citoesqueléticas en confinamiento e interacciones citoesqueleto-membrana.

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Bioingeniería Número 177 Transferencia de emulsión cDICE GUVs reconstitución de abajo hacia arriba actina fasina
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