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Journal / Biology / 原代人鼻上皮细胞：精准医学背景下的生物样本库…
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原代人鼻上皮细胞：精准医学背景下的生物样本库
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原代人鼻上皮细胞：精准医学背景下的生物样本库

Article DOI: 10.3791/63409-v • 08:35 min • April 22nd, 2022
April 22nd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2,3
1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées, Assistance Publique Hôpitaux de Paris

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在这里，我们描述了原代人鼻上皮（HNE）细胞在气液界面处的分离，扩增和分化，以及允许成功冷冻和解冻扩增的HNE的生物样本库方案。该方案分析了不同调节剂处理下分化HNE细胞的电生理特性和CFTR相关氯离子分泌校正。

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生物学，第182期，囊性纤维化，原发性人鼻上皮细胞，冷冻保存，cftr调节剂，生物样本库，精准医学
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