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Journal / Biology / Primære humane neseepitelceller: Biobanking i…
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Primære humane neseepitelceller
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Primære humane neseepitelceller: Biobanking i sammenheng med presisjonsmedisin

Article DOI: 10.3791/63409-v • 08:35 min • April 22nd, 2022
April 22nd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2,3
1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées, Assistance Publique Hôpitaux de Paris

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Her beskriver vi isolasjon, forsterkning og differensiering av primære humane neseepitelceller (HNE) ved luftvæskegrensesnittet og en biobankprotokoll som gjør det mulig å fryse og deretter tine forsterket HNE. Protokollen analyserer elektrofysiologiske egenskaper til differensierte HNE-celler og CFTR-relatert kloridsekresjonskorreksjon ved ulike modulatorbehandlinger.

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Biologi Utgave 182 cystisk fibrose primære humane neseepiteringsceller kryopreservering cftrmodulatorer biobanking presisjonsmedisin
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