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Journal / Biochemistry / Motilitet af enkeltmolekyler og klynger af tovejs …
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Biochemistry

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JoVE Journal Biochemistry
Motilitet af enkeltmolekyler og klynger af tovejs kinesin-5 Cin8 renset fra S. cerevisiae-celler
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Motilitet af enkeltmolekyler og klynger af tovejs kinesin-5 Cin8 renset fra S. cerevisiae-celler

Article DOI: 10.3791/63425-v • 10:46 min • February 2nd, 2022
February 2nd, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1Department of Chemistry, Ben-Gurion University of the Negev, Israel, 2Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Israel

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Den tovejs mitotiske kinesin-5 Cin8 akkumuleres i klynger, der splittes og fusionerer under deres bevægelighed. Akkumulering i klynger ændrer også Cin8's hastighed og retningsvirkning. Her beskrives en protokol for motilitetsassays med oprenset Cin8-GFP og analyse af bevægelige egenskaber af enkeltmolekyler og klynger af Cin8.

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Biokemi udgave 180 kinesin Cin8 enkeltmolekylemotilitet mikrotubuli tovejsmotilitet
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