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Journal / Biochemistry / Enkeltmolekyleanalyse af Sf9 rensede…
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JoVE Journal Biochemistry
Enkeltmolekyleanalyse af Sf9 rensede superprocessive Kinesin-3-familiemotorer
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Enkeltmolekyleanalyse af Sf9 rensede superprocessive Kinesin-3-familiemotorer

Article DOI: 10.3791/63837-v • 08:16 min • July 27th, 2022
July 27th, 2022 •
1,2, 1, 2, 1
1Discipline of Biological Engineering, Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics, Sambalpur University

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Denne undersøgelse beskriver oprensning af KIF1A (1-393LZ), et medlem af kinesin-3-familien, ved hjælp af Sf9-baculovirusekspressionssystem. In vitro enkeltmolekyle og multimotorisk glideanalyse af disse oprensede motorer udviste robuste bevægelighedsegenskaber, der kan sammenlignes med motorer fra pattedyrcellelysat. Således er Sf9-baculovirus-systemet egnet til at udtrykke og rense motorprotein af interesse.

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