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JoVE Journal Genetics
Påvisning af postreplikation af huller akkumulering og reparation i humane celler ved hjælp af DNA-fiberassayet
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Påvisning af postreplikation af huller akkumulering og reparation i humane celler ved hjælp af DNA-fiberassayet

Article DOI: 10.3791/63448-v • 10:32 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 2, 1
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 2Division of Oncology, Department of Internal Medicine, Washington University in St. Louis
* These authors contributed equally

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Her beskriver vi to modifikationer af DNA-fiberassayet for at undersøge enkeltstrengede DNA-huller i replikerende DNA efter induktion af læsioner. S1-fiberassayet muliggør detektion af postreplikative huller ved hjælp af den ssDNA-specifikke S1-endonuklease, mens gap-filling-analysen muliggør visualisering og kvantificering af gap-reparation.

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Genetik udgave 180 postreplikativt enkeltstrenget DNA-hul mellemrumsfyldning reparation efter replikation DNA-replikation DNA-fiberassay S1-nuklease repriming
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