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Journal / Biochemistry / DNA帘法破译组蛋白组装的分子机制
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Biochemistry

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JoVE Journal Biochemistry
DNA帘法破译组蛋白组装的分子机制
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DNA帘法破译组蛋白组装的分子机制

Article DOI: 10.3791/63501-v • 06:32 min • March 9th, 2022
March 9th, 2022 •
1, 1, 1, 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

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Summary
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DNA curtain 是一种高通量单分子成像技术，为实时可视化各种蛋白质-DNA 相互作用提供了一个平台。本方案利用DNA帘技术研究了含有裂 殖酵 母的溴结构域AAA+ ATP酶Abo1的生物学作用和分子机制。

Tags

分子机制、组蛋白组装、DNA 幕技术、染色质、真核 DNA、动态改变、细胞周期阶段、环境刺激、基因组完整性、表观遗传调控、DNA 代谢反应、复制、转录、修复、组蛋白伴侣、核小体、单分子成像技术、DNA 幕、脂质流动性、微流体、全内反射荧光显微镜 （TIRFM）、蛋白质-DNA 相互作用、Abo1、裂殖酵母含溴结构域的 AAA+ ATP 酶
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