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JoVE Journal Medicine
Subkonjunktival administration af adeno-associerede virusvektorer i smådyrsmodeller
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Subkonjunktival administration af adeno-associerede virusvektorer i smådyrsmodeller

Article DOI: 10.3791/63532-v • 06:16 min • March 16th, 2022
March 16th, 2022 •
1,3, 2, 1,2
1Department of Ophthalmology, University of North Carolina at Chapel Hill, 2Gene Therapy Center, University of North Carolina at Chapel Hill, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

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I dette manuskript demonstreres subkonjunktival injektion som en gyldig vektorleveringsmetode for okulært væv hos mus ved hjælp af et injektionssystem bestående af en infusions- / tilbagetrækningssprøjtepumpe og en gastæt aftagelig sprøjte kombineret med mikroinjektionsnåle. Dette injektionssystem kan også tilpasses til andre intraokulære administrationsveje.

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Medicin udgave 181 Subkonjunktival injektion Genterapi Virus Adeno-associeret virus (AAV) Øje Hornhinde Mus
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