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Journal / Biochemistry / 포유류 세포에서 유비퀴틴화된 p53 단백질의…
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Biochemistry

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JoVE Journal Biochemistry
포유류 세포에서 유비퀴틴화된 p53 단백질의 정제
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포유류 세포에서 유비퀴틴화된 p53 단백질의 정제

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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프로토콜은 p53 종양 억제 단백질을 예로 사용하여 포유류 세포로부터 유비퀴틴화된 단백질을 정제하는 단계별 방법을 설명합니다. 유비퀴틴화된 p53 단백질은 엄격한 비변성 및 변성 조건 하에서 세포로부터 정제하였다.

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