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Journal / Biochemistry / Um ensaio de recuo de molécula única otimizado…
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Biochemistry

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JoVE Journal Biochemistry
Um ensaio de recuo de molécula única otimizado para quantificação da fosforilação de proteínas
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Journal (Biochemistry)
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
Journal (Biochemistry)
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
 
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Um ensaio de recuo de molécula única otimizado para quantificação da fosforilação de proteínas

Article DOI: 10.3791/63665-v • 07:45 min • June 6th, 2022
June 6th, 2022 •
1, 1, 1, 2, 2, 1, 2,3, 1,3
1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center

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O presente protocolo descreve a preparação da amostra e a análise de dados para quantificar a fosforilação proteica usando um ensaio de recuo de molécula única (SiMPull).

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Bioquímica Edição 184
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