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Etablering af humane lungeorganoider og proksimal differentiering for at generere modne luftvejsorganoider
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Etablering af humane lungeorganoider og proksimal differentiering for at generere modne luftvejsorganoider

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Protokollen præsenterer en metode til at udlede humane lungeorganoider fra primære lungevæv, udvide lungeorganoiderne og inducere proksimal differentiering for at generere 3D- og 2D-luftvejsorganoider, der trofast phenocopy det menneskelige luftvejsepitele.

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Biologi udgave 181
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