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성숙한 기도 오가노이드를 생성하기 위해 인간 폐 오가노이드 및 근위 분화 확립

Trypsinizing and Subculturing Mammalian Cells

Journal (Biology)

Trypsinizing and Subculturing Mammalian Cells

Preparation and In Vitro Differentiation of Mesenchymal Stem Cell-Encapsulated Hydrogels for Bone Formation (Video) | JoVE

Journal (Biology)

Preparation and In Vitro Differentiation of Mesenchymal Stem Cell-Encapsulated Hydrogels for Bone Formation (Video) | JoVE

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성숙한 기도 오가노이드를 생성하기 위해 인간 폐 오가노이드 및 근위 분화 확립

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022

March 23rd, 2022 •

Cun Li^1,2, Man Chun Chiu^1,2, Yifei Yu^1,2, Xiaojuan Liu^1,2, Ding Xiao^1,2, Jingjing Huang^1,2, Zhixin Wan^1,2, Jie Zhou^1,2,3

¹Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, ²Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, ³State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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이 프로토콜은 원발성 폐 조직으로부터 인간 폐 오가노이드를 유도하고, 폐 오가노이드를 확장시키고, 근위 분화를 유도하여 인간 기도 상피를 충실하게 표현 복사하는 3D 및 2D 기도 오가노이드를 생성하는 방법을 제시한다.

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