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JoVE Journal Neuroscience
In situ Hibridación combinada con inmunohistoquímica en embriones crioseccionados de pez cebra
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In situ Hibridación combinada con inmunohistoquímica en embriones crioseccionados de pez cebra

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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Este protocolo describe cómo obtener imágenes combinando hibridación in situ e inmunohistoquímica de secciones embrionarias de pez cebra. La hibridación in situ se realizó antes de la criosección, seguida de tinción de anticuerpos. Es útil detectar los patrones de expresión de dos genes en el pez cebra si hay escasez de anticuerpos.

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Este mes en JoVE Número 181 Hibridación in situ inmunohistoquímica pez cebra ARNm anticuerpo proteína
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