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Vezeltype en subcellulair-specifieke analyse van lipidedruppelgehalte in skeletspieren
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Vezeltype en subcellulair-specifieke analyse van lipidedruppelgehalte in skeletspieren

Article DOI: 10.3791/63718-v • 11:50 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1, 1,2
1Endocrinology, Diabetes and Nutrition Unit, Institute of Experimental and Clinical Research, Medical Sector, Université Catholique de Louvain, 2Departamento de Fisiología, Facultad de Biología, Universidad de Sevilla

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Toenemend bewijs geeft aan dat overmatige infiltratie van lipiden in de skeletspieren resulteert in lipotoxiciteit en diabetes. Hier presenteren we een compleet protocol, inclusief weefselverwerking, kleuring met Bodipy, beeldacquisitie en analyse, om de grootte, dichtheid en subcellulaire verdeling van lipidedruppels op een vezeltypespecifieke manier te kwantificeren.

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Geneeskunde Nummer 184
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