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Biochemistry

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JoVE Journal Biochemistry
En massespektrometribaseret tilgang til identifikation af fosfoproteinphosphataser og deres interaktionorer
Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS (Video) | JoVE
Journal (Biochemistry)
Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS (Video) | JoVE
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Journal (Biochemistry)
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
 
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En massespektrometribaseret tilgang til identifikation af fosfoproteinphosphataser og deres interaktionorer

Article DOI: 10.3791/63805-v • 10:17 min • April 29th, 2022
April 29th, 2022 •
1, 1,2
1Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

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Her præsenterer vi en protokol til berigelse af endogene phosphoproteinphosphataser og deres interagerende proteiner fra celler og væv og deres identifikation og kvantificering ved massespektrometribaseret proteomik.

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Biokemi udgave 182
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