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Journal / Biology / Direkte måling af kræfter i rekonstituerede…
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JoVE Journal Biology
Direkte måling af kræfter i rekonstituerede aktive mikrotubulusbundter
Dose Calibration of the Transmission Electron Microscope for TEM and Scanning TEM (STEM) Imaging Modes Using a Machine-Vision Approach (Video) | JoVE
Journal (Biology)
Dose Calibration of the Transmission Electron Microscope for TEM and Scanning TEM (STEM) Imaging Modes Using a Machine-Vision Approach (Video) | JoVE
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Journal (Biology)
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
 
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Direkte måling af kræfter i rekonstituerede aktive mikrotubulusbundter

Article DOI: 10.3791/63819-v • 07:47 min • May 10th, 2022
May 10th, 2022 •
*1, *1, 1
1Department of Biological Sciences and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
* These authors contributed equally

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Her præsenterer vi en protokol til rekonstituering af mikrotubulusbundter in vitro og direkte kvantificering af de kræfter, der udøves i dem ved hjælp af samtidig optisk fangst og total intern refleksionsfluorescensmikroskopi. Dette assay muliggør måling på nanoskala-niveau af de kræfter og forskydninger, der genereres af proteinensembler inden for aktive mikrotubulinetværk.

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Biologi udgave 183 mikrotubuli optisk fangst mitose enkeltmolekyle spindel mekanik kinesin mikrotubuli-associerede proteiner fluorescensmikrosopi
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