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Generering af 3D-kugler og 2D luft-væske-grænsefladekulturer af humane inducerede pluripotente stamcelleafledte type 2 alveolære epitelceller…
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Developmental Biology
Developmental Biology
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Generering af 3D-kugler og 2D luft-væske-grænsefladekulturer af humane inducerede pluripotente stamcelleafledte type 2 alveolære epitelceller
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Article
DOI:
10.3791/63875-v
•
07:02 min
•
April 15th, 2022
Generering af 3D-kugler og 2D luft-væske-grænsefladekulturer af humane inducerede pluripotente stamcelleafledte type 2 alveolære epitelceller
April 15th, 2022
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Rhiannon B. Werder*1,2,3, Jessie Huang*1,2, Kristine M. Abo1,2, Olivia T. Hix1,2, Kasey Minakin1,2, Konstantinos-Dionysios Alysandratos1,2, Carly Merritt1,2, Kayleigh Berthiaume1,2, Andrea B. Alber1,2, Claire L. Burgess1,2, Darrell N. Kotton*1,2, Andrew A. Wilson*1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Den foreliggende protokol beskriver humant inducerede pluripotente stamcelleafledte type 2 alveolære epitellignende celler (iAT2s). Disse celler kan dyrkes som selvfornyende kugler i 3D-kultur eller tilpasses luft-væske-interface (ALI) kultur.